all of your advice given on this topic or on any other topic is always welcomed!
I'm going to give your advice a try to see what happens.
I wonder if adding some yeast nutrient before or after freezing would help with with the cell survival rate???
I always make my starters using yeast nutrient, and i take my yeast samples from the flask while the starter's fermentation is at it's highest peak of fermentation by top cropping it. I'm sure that some of the nutrients are coming along into the vial with the yeast.
Wottaguy, you really want to babysit that starter don't you? 10-14 days? I don't have the patients.
Legman...YES! Yeastizes are my friends...LOL
Well...I figure that i'll be stepping up the starter to grow it up big enough to pitch.(I like BIG starters). During this process, I'll also have to cool off the fermented wort from the starters so that the yeast drops out...decant...warm it to room temp...make another starter medium..I usually cool the starter off in the fridge for 24 hours, then make more starter wort the next morning. When I cool the starter wort off, I then take the cooled off flask and the settled yeast out of the fridge and let it warm up. when all is ready, i decant the beer from the flask, then introduce the newly cooled off starter wort into the flask. I also aerate the new wort before transferring it to the flask. I actually have a new starter in progress cooling off as we speak! (brewing tomorrow).
Hope all this makes sense....LOL!
Thanks Guys...I appreciate your comments and ideas!
Now get out there and make some beer...!!!